The stress response may enable survival in addition to increasing the virulence potential of the bacterium 2. coli, like the pathogenic variants, respond to sublethal environmental triggers by means of a stress response. It is a model organism for biological research due to its non-pathogenic properties, easy handling, and a wide nutritional palate. coli) is found as commensal member of the mammalian gut microbiome, and, in the case of some pathotypes, as an enteric pathogen 1, in addition to being omnipresent in soil, water and plants. Identifying how they respond to distinct challenges will elucidate common and distinct processes that underlie physiological adaptation to challenging environments.Įscherichia coli ( E. Bacterial regulatory proteins controlling the flow of genetic information often respond to environment signals by fine-tuning their transcriptional network. Bacterial gene expression is regulated by two tightly coupled processes, transcription and translation, that allow bacteria to survive numerous environmental insults. Regulation of gene expression is important to organisms, as it allows them to survive fluctuating and stressful environments without changes in gene content. These networks validate known gene interactions and provide new insights into genes mediating transcriptional regulation in specific stress environments, thus allowing for in silico hypothesis generation. In addition, we also studied the link between transcription factors and their differentially regulated targets to understand the regulatory mechanisms involved.
The modules were analysed for identification of hub genes, enrichment of biological processes and transcription factors. Based on the gene co-expression networks, genes with similar expression profiles were clustered into modules. Gene co-expression networks were generated and analyzed via Weighted Gene Co-expression Network Analysis (WGCNA).
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